Plasmid

Part:BBa_K4404009:Design

Designed by: Julia Fricke   Group: iGEM22_Goettingen   (2022-10-09)

acsB1 from A. woodii under the control of a PbgaL promoter from C. perfringens


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1599
    Illegal EcoRI site found at 1944
    Illegal EcoRI site found at 2232
    Illegal EcoRI site found at 2679
    Illegal EcoRI site found at 6154
    Illegal XbaI site found at 6181
    Illegal PstI site found at 6044
    Illegal PstI site found at 6220
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1599
    Illegal EcoRI site found at 1944
    Illegal EcoRI site found at 2232
    Illegal EcoRI site found at 2679
    Illegal EcoRI site found at 6154
    Illegal NheI site found at 2286
    Illegal NheI site found at 6399
    Illegal PstI site found at 6044
    Illegal PstI site found at 6220
    Illegal NotI site found at 6123
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1599
    Illegal EcoRI site found at 1944
    Illegal EcoRI site found at 2232
    Illegal EcoRI site found at 2679
    Illegal EcoRI site found at 6154
    Illegal BglII site found at 6208
    Illegal BamHI site found at 2270
    Illegal BamHI site found at 6175
    Illegal XhoI site found at 105
    Illegal XhoI site found at 6212
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1599
    Illegal EcoRI site found at 1944
    Illegal EcoRI site found at 2232
    Illegal EcoRI site found at 2679
    Illegal EcoRI site found at 6154
    Illegal XbaI site found at 6181
    Illegal PstI site found at 6044
    Illegal PstI site found at 6220
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1599
    Illegal EcoRI site found at 1944
    Illegal EcoRI site found at 2232
    Illegal EcoRI site found at 2679
    Illegal EcoRI site found at 6154
    Illegal XbaI site found at 6181
    Illegal PstI site found at 6044
    Illegal PstI site found at 6220
    Illegal NgoMIV site found at 3593
    Illegal NgoMIV site found at 5500
    Illegal NgoMIV site found at 5510
    Illegal NgoMIV site found at 5636
    Illegal AgeI site found at 215
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 5252
    Illegal SapI.rc site found at 5607
    Illegal SapI.rc site found at 5978


Design Notes

It is important to produce inserts with specific restriction enzymes in order to secure a robust implementation into a vector system.


Source

The constitutive promoter originated from C. perfringens, while acsB1 was amplified from genomic DNA of Acetobacterium woodii with specific primers by PCR. The pMTL83151 based on the ClosTron plasmid system and was modified by Prof. Peter Dürre (department of microbiology and biotechnology).

References